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The Virtual Microscope is a Java application that supports interactive viewing of high-resolution, multi-dimensional image datasets from various microscopes. We Currently support data from a Philips Environmental Scanning Electron Microscope (ESEM), and a Fluorescence Light Microscope. Interface Controls The interface provides a simulation of our actual microscope interface(s). Controls common to all datasets include brightness, magnification (usually up to 1800x), navigation, measurement, and annotation. SEM samples include a contrast control, and some also include a focus control. The navigation and magnification controls allow the user to explore any point of Interest on the sample. This differs from other virtual microscopes that have been produced which provide only a few 'hot spots' that can be magnified and explored. Focus The focus control is a real focal plane adjustment instead of a blurring of the image. Our automated data Capture software (see below) allows us to capture an arbitrary number of focal planes. Thus, the focus control on the Virtual Microscope allows the user to glide through those planes interactively, bringing items closer and farther into focus. This is particularly useful with thick specimens viewed in the SEM. Annotations We have included a full set of specimen annotation tools so that experts and laypeople alike can mark up the datasets for future reference. These annotations are useful for teaching Details about the data, as well as for allowing teachers to Ask students to identify data features as homework. The annotations can be saved and reloaded as XML files.

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