Hyper32 Is a programm for the analysis of enzyme-kinetic data.
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video prev next Is a program for the analysis of enzyme-kinetic data. The velocity (v) of enzyme-catalyzed reactions is hyperbolically related to the substrate concentration () through the Michaelis-Menten equation: v = Vmax./(Km ) where Vmax and Km are kinetic constants characteristic of the particular enzyme and substrate. These constants are estimated in experiments in which is varied and v measured. They are usually extracted by some linear transformation of the data such as the Lineweaver-Burk or Hanes plots. These transformations are unsatisfactory as they can result in artificial weighting of the data leading to erroneous estimates of Vmax and Km.

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