Serial Cloner free download, Serial Cloner download on software download

Provides tools with an intuitive interface that assists you in DNA cloning, sequence analysis and visualization Serial Cloner is a Molecular Biology software that provides tools with an intuitive interface to assists you in DNA cloning, sequence analysis and visualization.Serial Cloner now handles Features/Annotations, which has been the most requested enhancement. User can annotate any sequence portion or use the automatic scan to detect common features.Serial Cloner can read and write DNA Strider-compatible files and also import or export files in FASTA format. Also new in this version is the possibility to read ApE and VectorNTI files with their features as well as pDRAW32 sequences. Genbank/EMBL files can also be imported or read from internet using the included web interface.Powerful graphical display tools and simple interfaces help the analysis and construction steps in a very intuitive way. All the tools you need to analyze and manipulate your sequences are available in an all-in-one-window concept. All key functions are accessible through a compact interface.Serial Cloner also lets you build text restriction map and quickly format it to add multi-frame translation or only show single cutters for example. The graphic map of Serial Cloner is really Graphic as you can easily select and extract a feature, a fragment or show single, double or multiple cutter all in the same window.Serial Cloner will assist you in setting-up new sub-cloning projects and in preparing the electronic versions of your constructs. In addition to the classical restriction maps - both graphic and text-based - or site usage windows, you will be able to quickly set-up a PCR-based amplification or prepare synthetic adaptors. shRNA constructions based on pre-defined scaffolds are also automated.You can assemble fragments, obtained by PCR, adaptor/shRNA synthesis or simply by graphically selecting fragments between restriction sites. Just select, blunt if you need, and click the Ligate button.An additional interface allows easy Gateway cloning for both BP and LR reactions. Finally, Serial Cloner provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server and extract a consensus a virtual cutter, a web browser with instant import of NCBI/EMBL entries and a silent restriction map generator.Serial Cloner also allows to quickly prepare Fasta-formatted text or files to submit a multi-alignement request to Clustal. What's New in This Release: · Serial Cloner now handles Features/Annotations. Features can be added manually or by scanning (see bellow). · A new panel in the Sequence Window to manage Features/Annotations · A scan function (available both in the Sequence Window and in the Toolbar) to automatically detect Features. At the moment, it uses an internal database. The next version of Serial Cloner will allow to define user lists. · Automatic Scan available directly in the Graphic Map (available from the Toolbar or from the menu) · Add a Feature after selection of a segment in the Graphic map. · Selection of the feature sequence in the Sequence Window when double clicking in the feature name in the Graphic map. · Assemble and save a multi-Sequence Fasta-formated file containing all currently opened sequence (useful for multi-sequence alignment (e.g. CLUSTAL) submission) · Assemble and copy to the Clipboard a multi-Sequence Fasta-formated text containing all currently opened sequence (useful for multi-sequence alignment (e.g. CLUSTAL) submission) · Automatic recovery of unsaved sequences in (hopefully rare) case of crashing. · Scrolling in sequences and graphic map windows using mouse wheel. · User can modify the width of the Graphic Map window · The size of the selected fragment is now displayed in the Graphic Map · Drag and reposition the restriction sites and features names in the Graphic Map. · Serial Cloner now tries to guess whether a degenerate sequence file is being opened. · Import Vector NTI sequences with their features. · Import ApE sequences with their features. · Import Genbank Sequences (from a file) with their features. · Several shortcut have been added for the major functions (Import, degenerate sequence, etc.) · Use alt-click to reveal alternative commands now available in the Toolbar (New Degenerate Sequence, Import, Interactive site usage). · Automatic internet proxy detection using system settings (seems not to work if using a .pac file under MacOSX). · MacOSX : a windows proxy (small black dot displayed in the red "close window" ball) is shown when the sequence has been modified and has not been saved. · Sequence Names can be modified directly in the Sequence Window by double-clicking on the name or using a contextual menu available by CTRL-Clicking (or Right-clicking) on the Sequence name. · Additional parameters can be set-up in the preference window (Internet Proxy management, Warning when importing non-Serial cloner files). · Extended contextual menu in the Graphic Map window · Correct Sequence Format indication displayed in the Sequence window when opening a sequence in a Genbank, EMBL, DDBJ, VNTI, pDRAW32 or ApE. · Import of NCBI sequences using the Web Access window (and the automatic parsing button) has been improved · Alignment at NCBI using the BLAST 2 sequence software is re-established. · Improve import of sequence that nearly, but not fully, respect the layout of NCBI sequences (using the Web Access windows). · Change the interface of the window used to announce the availability of a new version. · Handling of certain type IIs restriction enzymes like XXXXX,-a,-b or XXXXX,-a,+b. · A crash occurring when changing CW/CCW without any selection done in Graphic Map. · A bug in PCR occurring when amplifying very long sequences (more then 32 k) · MacOSX : A bug in the behavior of the drawer window of the 'Manage Restriction Enzyme' window. · A bug in the Fragment selection of the 'Build a construct' window. · A rare bug in the Local Alignment that was deleting a portion of the aligned output · A pop-up windows is no longer displayed indicating that the sequence was locked showed up when using the 'Save as.. ' command. · The calculation of the % of aligned nucleotide in the Local Alignment Window has been corrected. · A bug preventing Serial Cloner to import Fasta files containing accentuated characters in the header. · MacOSX : An interface glitch in the 'Find' drawers of the Sequence Window · WINDOWS : a problem with 'paste commands' in the find window. · A bug occurring when back selecting DNA from the translation field if the DNA was translated from the minus strand. · A bug affecting degenerated restriction enzymes in the 'Build a Construct' Window.

Serial Cloner Related Software