GelEval GelEval - a tool for molecular biologists for quantifying images of electrophoresis gels
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molecular macro Biology molecular biology electrophoresis gel quantify image

GelEval Description

GelEval - a tool for molecular biologists for quantifying images of electrophoresis gels GelEval is a Macintosh software tool designed primarily for molecular biologists for quantifying images of electrophoresis gels (e.g. RNA, DNA or protein gels or Western blots).GelEval is a useful tool that allows you to measure the densities of gel bands or to scan entire gel lanes.Starting from the scanned image of a gel, GelEval allows you to delimit the boundaries of individual bands and lanes. The total density of individual bands can then be measured. If your gel contains known standards, line-fitting can be applied to express band intensity relative to the standards. Alternatively, lanes can be scanned to give a graphical representation of intensity as you move from top to bottom of the delimited region.These features are presented with an easy-to-use interface that uses intuitive Macintosh actions. All your measurements can be saved to a custom quantification file for future reference.Here are some key features of "GelEval":· Measure the density of bands on a gel.· Compare band densities to known standards using line-fitting algorithms.· Subtract background density in 3 different ways: as a constant 'area' background from somewhere else in the image, as a varying 'gradient' from top to bottom of the gel, as a 'local' background derived from the edges of individual bands· Show you how setting the background in different ways changes the calculated band density.· Creates graphs of the density individual bands or of whole lanes.· Maintain a notepad where you can enter experimental and analysis details. The notepad is indexed by Spotlight, making it easy for you to find the relevant files.· Plus many other user-friendly features.· GelEval is under continued expansion and revision.Limitations:· 20 uses trial What's New in This Release: · Fixed a bug where double-clicking graph axes to set a new maximum created a new graph window. · Changed the axis parameter sheet so you can change all graph axis parameters: axis minimum and maximum, position and spacing of major ticks, and the number of minor ticks. · Fixed a bug where in Leopard, the Paste-At-Point cursor was drawn very large. · Changed to the Leopard-compatible Zonic KRM 1.07.

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