Bio::MCPrimers Bio::MCPrimers is a Perl module to create molecular cloning PCR primer pairs for a given gene.
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Perl module molecular cloning molecular cloning gene cloning

Bio::MCPrimers Description

Bio::MCPrimers is a Perl module to create molecular cloning PCR primer pairs for a given gene. Bio::MCPrimers is a Perl module to create molecular cloning PCR primer pairs for a given gene so that the gene can be directionally inserted into a vector. Solver is generic, restriction enzymes and their order in the vector are specified in the caller.XPORT SUBROUTINESsub find_mc_primers $orf, # ATGC string (I use 21 NT upstream, 200 NT downsteam) $flag_hr, # anonymous hash reference to flags from mcprimers.pl $pr3_hr, # hash reference to Primer3 Boulder file tags $ecut_loc_ar, # enzyme cut location array reference from caller $vcut_loc_ar, # vector cut location array reference from caller @re # array of restriction enzyme strings from callerNot explicitily exported. Use Bio::MCPrimers::find_mc_primersSee mcprimers.pl for an example of use and front-end.INSTALLATION MCPrimers.pm - Place into lib/Bio/MCPrimers.pm CloningVector.pm - Place into lib/Bio/Data/Plasmid/CloningVector.pm Vector files - Make vector file directory accessable mcprimers.pl - Place in a directory where it can be accessed mcprimers_gui.pl - Place in a directory where it can be accessed mcprimers.acd - Put in acd directory for EMBOSS Only checked with acdvalidate MCPrimers_manual.doc - User documentation MCPRIMER_DIR - Set this environment variable to point to the directory containing mcprimers.pl PRIMER3_DIR - Set environment variable to point to Primer3 executable directory. MSWindows - use primer3.exe Other - use primer3_core Requirements: · Perl

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